YSI 6 series User Manual download pdf (Page 17)

CHLOROPHYLLCALIBRATIONTIPS

BEFORE YOU BEGIN: Make sure that the sonde firmware is version 3.08 or higher. Upgrade at

www.ysi.com

SET UP & OPERATION

• Confirm that the wiper is parking correctly and reverses direction.

• Clean the optics of dirt, fingerprints, etc.

• In Unattended Studies, sensor stability can be improved by increasing the probe’s sampling

frequency. Go the sonde’s Advanced Menu > Data Filter > Time Constants and change the Chl

time to 30 seconds.

Note: Battery life will be slightly reduced.

• Make sure that the back edges of the chlorophyll wiper remain painted black to eliminate stray

light reflection. This applies to the anti-fouling copper alloy wipers too.

• Always calibrate in the long calibration cup or use a clean sensor guard or the anti-fouling

copper alloy guard.

• Never calibrate the chlorophyll probe in direct or bright sunlight. A darkened environment is

optimum.

CALIBRATION PROCESS

1. Set the sonde’s Report output to display both the ug/l and RFU units. Mix your calibration

standard and set aside so that both the standard and the sonde are at ambient temperature. Verify

that the optics are clean. And finally, test the operation of the wiper. Confirm that the wipers on

all optical probes park correctly and that the pads are in good condition before proceeding.

2. ONE- POINT CALIBRATION: The chlorophyll sensor must be given at least a one-point

calibration before use. Fill the sonde’s calibration cup (black bottom) with enough distilled or

deionized water to completely cover the temperature and conductivity sensors. Run the wiper at

least once, and wait 30 seconds for the reading to stabilize after the last wiper has stopped. You

will enter the number zero for all one point chlorophyll calibrations! If the one point calibration

option is selected, the factory default calibration slope will be used for the second point.

3. TWO- POINT CALIBRATION: If you choose the two-point calibration, then the first point will

be zero as described above. The second point entry will come from the tables for the dye used to

prepare your standard. The instructions and calibration tables can be found in the Principles of

Operations section of your manual. Measure the temperature of the dye standard and use the

corresponding ug/l value in the table. Enter this number and run the wiper. Wait at least 30

seconds before you accept the calibration point. Visually inspect the probe face to make sure it is

bubble-free. The second point can also be a sample of water with a known chlorophyll value.

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YSI 6 series User Manual Page 17